Virus:Article Title: TfR1 facilitates influenza virus endocytosis and uncoating by interacting with NA and M1 via extracellular and intracellular domains
Article Snippet: .. Antibodies and Proteins: Mouse anti-CD71 monoclonal antibody (clone 3C11F11, Proteintech, #66180–1-Ig), Rabbit anti-SH3 BP4 polyclonal antibody (Proteintech, #17691–1-AP), Mouse anti-GAPDH monoclonal antibody (1E6D9, Proteintech, #60004–1-Ig), Mouse anti-NP (Influenza A Virus) monoclonal antibody (9G8, Santa Cruz, #sc-101352), Mouse anti-HA (H1N1) monoclonal antibody (2D10C9E2, Sino Biological, #11052-MM06), Mouse anti-NA (H1N1) monoclonal antibody (GT288, GeneTex, t#GTX629696), Rabbit anti-matrix protein (M1, H1N1) polyclonal antibody (GeneTex, #GTX125928), Rabbit anti-matrix protein (M2, H1N1) polyclonal antibody (GeneTex, Inc (GeneTex, #GTX125951), HDAC6 Rabbit mAb (D2E5, Cell Signaling Technology, #7558), Ubiquitin Rabbit mAb (E4I2J,Cell Signaling Technology, #43124), DYKDDDDK tag mouse Monoclonal antibody (Proteintech, #66008–4-Ig), His-Tag Mouse Monoclonal antibody (Proteintech, #66005–1-Ig), Mouse FITC anti-human CD71 monoclonal Antibody (CY1G4, BioLegend, #334104), CD71 Mouse Monoclonal Antibody (OKT-9, Thermo Fisher, #14-0719-82), Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology, #7074), Rabbit anti-Biotin polyclonal antibody (Bioss, #bs-0311R), Anti-mouse IgG HRP-linked Antibody (Cell Signaling Technology, #7076), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (Abcam, #ab150077), Goat Anti-Rabbit IgG H&L Alexa Fluor 555 (Abcam, #ab150078), Goat Anti-Rabbit IgG H&L Alexa Fluor 594 (Abcam, #ab150080), Goat Anti-Mouse IgG H&L Alexa Fluor 488 (Abcam #ab150113), Goat Anti-Mouse IgG H&L Alexa Fluor 647 (Abcam, #ab150115), Alexa Fluor 488 conjugated-Streptavidin (Yeasen Biotechnology, #35103ES60), Streptavidin Agarose Resin 6FF (Yeasen Biotechnology, #20512ES60). .. Transferrin Receptor/TFR1/CD71 Protein, Human, Recombinant (ECD, His Tag), HPLC-verified (Sino Biological, Cat#11020-H07H), Influenza A H1N1 (A/WSN/1933) Hemagglutinin/ HA Protein (His Tag) (Sino Biological, Cat#11692-V08H), Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/ M1 Protein (His Tag), (Sino Biological, Cat#40010-V07E).
Article Title: TfR1 facilitates influenza virus endocytosis and uncoating by interacting with NA and M1 via extracellular and intracellular domains.
Article Snippet: .. Antibodies and Proteins: Mouse anti-CD71 monoclonal antibody (clone 3C11F11, Proteintech, #66180–1-Ig), Rabbit anti-SH3 BP4 polyclonal antibody (Proteintech, #17691–1-AP), Mouse anti-GAPDH monoclonal antibody (1E6D9, Proteintech, #60004–1-Ig), Mouse anti-NP (Influenza A Virus) monoclonal antibody (9G8, Santa Cruz, #sc-101352), Mouse anti-HA (H1N1) monoclonal antibody (2D10C9E2, Sino Biological, #11052-MM06), Mouse anti-NA (H1N1) monoclonal antibody (GT288, GeneTex, t#GTX629696), Rabbit anti-matrix protein (M1, H1N1) polyclonal antibody (GeneTex, #GTX125928), Rabbit anti-matrix protein (M2, H1N1) polyclonal antibody (GeneTex, Inc (GeneTex, #GTX125951), HDAC6 Rabbit mAb (D2E5, Cell Signaling Technology, #7558), Ubiquitin Rabbit mAb (E4I2J,Cell Signaling Technology, #43124), DYKDDDDK tag mouse Monoclonal antibody (Proteintech, #66008–4-Ig), His-Tag Mouse Monoclonal antibody (Proteintech, #66005–1-Ig), Mouse FITC anti-human CD71 monoclonal Antibody (CY1G4, BioLegend, #334104), CD71 Mouse Monoclonal Antibody (OKT-9, Thermo Fisher, #14-0719-82), Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology, #7074), Rabbit anti-Biotin polyclonal antibody (Bioss, #bs-0311R), Anti-mouse IgG HRP-linked Antibody (Cell Signaling Technology, #7076), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (Abcam, #ab150077), Goat Anti-Rabbit IgG H&L Alexa Fluor 555 (Abcam, #ab150078), Goat Anti-Rabbit IgG H&L Alexa Fluor 594 (Abcam, #ab150080), Goat AntiMouse IgG H&L Alexa Fluor 488 (Abcam #ab150113), Goat Anti-Mouse IgG H&L Alexa Fluor 647 (Abcam, #ab150115), Alexa Fluor 488 conjugated-Streptavidin (Yeasen Biotechnology, #35103ES60), Streptavidin Agarose Resin 6FF (Yeasen Biotechnology, #20512ES60). .. Transferrin Receptor/TFR1/CD71 Protein, Human, Recombinant (ECD, His Tag), HPLCverified (Sino Biological, Cat#11020-H07H), Influenza A H1N1 (A/WSN/1933) Hemagglutinin/ HA Protein (His Tag) (Sino Biological, Cat#11692-V08H), Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/ M1 Protein (His Tag), (Sino Biological, Cat#40010-V07E).
Ubiquitin Proteomics:Article Title: TfR1 facilitates influenza virus endocytosis and uncoating by interacting with NA and M1 via extracellular and intracellular domains
Article Snippet: .. Antibodies and Proteins: Mouse anti-CD71 monoclonal antibody (clone 3C11F11, Proteintech, #66180–1-Ig), Rabbit anti-SH3 BP4 polyclonal antibody (Proteintech, #17691–1-AP), Mouse anti-GAPDH monoclonal antibody (1E6D9, Proteintech, #60004–1-Ig), Mouse anti-NP (Influenza A Virus) monoclonal antibody (9G8, Santa Cruz, #sc-101352), Mouse anti-HA (H1N1) monoclonal antibody (2D10C9E2, Sino Biological, #11052-MM06), Mouse anti-NA (H1N1) monoclonal antibody (GT288, GeneTex, t#GTX629696), Rabbit anti-matrix protein (M1, H1N1) polyclonal antibody (GeneTex, #GTX125928), Rabbit anti-matrix protein (M2, H1N1) polyclonal antibody (GeneTex, Inc (GeneTex, #GTX125951), HDAC6 Rabbit mAb (D2E5, Cell Signaling Technology, #7558), Ubiquitin Rabbit mAb (E4I2J,Cell Signaling Technology, #43124), DYKDDDDK tag mouse Monoclonal antibody (Proteintech, #66008–4-Ig), His-Tag Mouse Monoclonal antibody (Proteintech, #66005–1-Ig), Mouse FITC anti-human CD71 monoclonal Antibody (CY1G4, BioLegend, #334104), CD71 Mouse Monoclonal Antibody (OKT-9, Thermo Fisher, #14-0719-82), Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology, #7074), Rabbit anti-Biotin polyclonal antibody (Bioss, #bs-0311R), Anti-mouse IgG HRP-linked Antibody (Cell Signaling Technology, #7076), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (Abcam, #ab150077), Goat Anti-Rabbit IgG H&L Alexa Fluor 555 (Abcam, #ab150078), Goat Anti-Rabbit IgG H&L Alexa Fluor 594 (Abcam, #ab150080), Goat Anti-Mouse IgG H&L Alexa Fluor 488 (Abcam #ab150113), Goat Anti-Mouse IgG H&L Alexa Fluor 647 (Abcam, #ab150115), Alexa Fluor 488 conjugated-Streptavidin (Yeasen Biotechnology, #35103ES60), Streptavidin Agarose Resin 6FF (Yeasen Biotechnology, #20512ES60). .. Transferrin Receptor/TFR1/CD71 Protein, Human, Recombinant (ECD, His Tag), HPLC-verified (Sino Biological, Cat#11020-H07H), Influenza A H1N1 (A/WSN/1933) Hemagglutinin/ HA Protein (His Tag) (Sino Biological, Cat#11692-V08H), Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/ M1 Protein (His Tag), (Sino Biological, Cat#40010-V07E).
Article Title: TfR1 facilitates influenza virus endocytosis and uncoating by interacting with NA and M1 via extracellular and intracellular domains.
Article Snippet: .. Antibodies and Proteins: Mouse anti-CD71 monoclonal antibody (clone 3C11F11, Proteintech, #66180–1-Ig), Rabbit anti-SH3 BP4 polyclonal antibody (Proteintech, #17691–1-AP), Mouse anti-GAPDH monoclonal antibody (1E6D9, Proteintech, #60004–1-Ig), Mouse anti-NP (Influenza A Virus) monoclonal antibody (9G8, Santa Cruz, #sc-101352), Mouse anti-HA (H1N1) monoclonal antibody (2D10C9E2, Sino Biological, #11052-MM06), Mouse anti-NA (H1N1) monoclonal antibody (GT288, GeneTex, t#GTX629696), Rabbit anti-matrix protein (M1, H1N1) polyclonal antibody (GeneTex, #GTX125928), Rabbit anti-matrix protein (M2, H1N1) polyclonal antibody (GeneTex, Inc (GeneTex, #GTX125951), HDAC6 Rabbit mAb (D2E5, Cell Signaling Technology, #7558), Ubiquitin Rabbit mAb (E4I2J,Cell Signaling Technology, #43124), DYKDDDDK tag mouse Monoclonal antibody (Proteintech, #66008–4-Ig), His-Tag Mouse Monoclonal antibody (Proteintech, #66005–1-Ig), Mouse FITC anti-human CD71 monoclonal Antibody (CY1G4, BioLegend, #334104), CD71 Mouse Monoclonal Antibody (OKT-9, Thermo Fisher, #14-0719-82), Anti-rabbit IgG, HRP-linked Antibody (Cell Signaling Technology, #7074), Rabbit anti-Biotin polyclonal antibody (Bioss, #bs-0311R), Anti-mouse IgG HRP-linked Antibody (Cell Signaling Technology, #7076), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (Abcam, #ab150077), Goat Anti-Rabbit IgG H&L Alexa Fluor 555 (Abcam, #ab150078), Goat Anti-Rabbit IgG H&L Alexa Fluor 594 (Abcam, #ab150080), Goat AntiMouse IgG H&L Alexa Fluor 488 (Abcam #ab150113), Goat Anti-Mouse IgG H&L Alexa Fluor 647 (Abcam, #ab150115), Alexa Fluor 488 conjugated-Streptavidin (Yeasen Biotechnology, #35103ES60), Streptavidin Agarose Resin 6FF (Yeasen Biotechnology, #20512ES60). .. Transferrin Receptor/TFR1/CD71 Protein, Human, Recombinant (ECD, His Tag), HPLCverified (Sino Biological, Cat#11020-H07H), Influenza A H1N1 (A/WSN/1933) Hemagglutinin/ HA Protein (His Tag) (Sino Biological, Cat#11692-V08H), Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/ M1 Protein (His Tag), (Sino Biological, Cat#40010-V07E).
Concentration Assay:Article Title: Bovine Ultra-Long CDR H3 Specific for Bovine Rotavirus Displays Potent Virus Neutralization and Therapeutic Effects in Infected Calves.
Article Snippet: .. The CoraLite®488-conjugated 6*His His-Tag Mouse Monoclonal antibody (Proteintech Group, Wuhan, China) was used as the secondary antibody at a concentration of 1:500; 2 mL of the working solution of the secondary antibody was added to each well, and the dish was incubated at room temperature for 1 h, and rinsed with PBST 3 times for 5 min each time. .. A 200 μL volume of a ready-to-use 4′, 6-diamidino-2-phenylindole (DAPI) (Beyotime Biotechnology, Shanghai, China) working solution was added to each well, and the dish was incubated at room temperature for 4 min, and rinsed with PBST 3 times for 5 min each time.
Incubation:Article Title: Bovine Ultra-Long CDR H3 Specific for Bovine Rotavirus Displays Potent Virus Neutralization and Therapeutic Effects in Infected Calves.
Article Snippet: .. The CoraLite®488-conjugated 6*His His-Tag Mouse Monoclonal antibody (Proteintech Group, Wuhan, China) was used as the secondary antibody at a concentration of 1:500; 2 mL of the working solution of the secondary antibody was added to each well, and the dish was incubated at room temperature for 1 h, and rinsed with PBST 3 times for 5 min each time. .. A 200 μL volume of a ready-to-use 4′, 6-diamidino-2-phenylindole (DAPI) (Beyotime Biotechnology, Shanghai, China) working solution was added to each well, and the dish was incubated at room temperature for 4 min, and rinsed with PBST 3 times for 5 min each time.
Article Title: An effective method to enhance transient gene expression by transfecting high-density CHO cells.
Article Snippet: .. Following three washes with TBST, the membranes were incubated with 1:10000 HRP-conjugated 6*His, His-Tag mouse Monoclonal antibody (proteintech, Catalog: HRP 66005) for 1.5 h. Protein bands were visualized using enhanced chemiluminescence substrate (Beyotime Biotech Co., Ltd., Shanghai, China), imaged with a Tannon gel imaging system, and quantified using Image J software. .. Statistical analysis was performed using SPSS 18.0 software.
Article Title: An effective method to enhance transient gene expression by transfecting high-density CHO cells
Article Snippet: .. Following three washes with TBST, the membranes were incubated with 1:10000 HRP-conjugated 6*His, His-Tag mouse Monoclonal antibody (proteintech, Catalog: HRP 66005) for 1.5 h. Protein bands were visualized using enhanced chemiluminescence substrate (Beyotime Biotech Co., Ltd., Shanghai, China), imaged with a Tannon gel imaging system, and quantified using Image J software. .. Statistical analysis was performed using SPSS 18.0 software.
Imaging:Article Title: An effective method to enhance transient gene expression by transfecting high-density CHO cells.
Article Snippet: .. Following three washes with TBST, the membranes were incubated with 1:10000 HRP-conjugated 6*His, His-Tag mouse Monoclonal antibody (proteintech, Catalog: HRP 66005) for 1.5 h. Protein bands were visualized using enhanced chemiluminescence substrate (Beyotime Biotech Co., Ltd., Shanghai, China), imaged with a Tannon gel imaging system, and quantified using Image J software. .. Statistical analysis was performed using SPSS 18.0 software.
Article Title: An effective method to enhance transient gene expression by transfecting high-density CHO cells
Article Snippet: .. Following three washes with TBST, the membranes were incubated with 1:10000 HRP-conjugated 6*His, His-Tag mouse Monoclonal antibody (proteintech, Catalog: HRP 66005) for 1.5 h. Protein bands were visualized using enhanced chemiluminescence substrate (Beyotime Biotech Co., Ltd., Shanghai, China), imaged with a Tannon gel imaging system, and quantified using Image J software. .. Statistical analysis was performed using SPSS 18.0 software.
Software:Article Title: An effective method to enhance transient gene expression by transfecting high-density CHO cells.
Article Snippet: .. Following three washes with TBST, the membranes were incubated with 1:10000 HRP-conjugated 6*His, His-Tag mouse Monoclonal antibody (proteintech, Catalog: HRP 66005) for 1.5 h. Protein bands were visualized using enhanced chemiluminescence substrate (Beyotime Biotech Co., Ltd., Shanghai, China), imaged with a Tannon gel imaging system, and quantified using Image J software. .. Statistical analysis was performed using SPSS 18.0 software.
Article Title: An effective method to enhance transient gene expression by transfecting high-density CHO cells
Article Snippet: .. Following three washes with TBST, the membranes were incubated with 1:10000 HRP-conjugated 6*His, His-Tag mouse Monoclonal antibody (proteintech, Catalog: HRP 66005) for 1.5 h. Protein bands were visualized using enhanced chemiluminescence substrate (Beyotime Biotech Co., Ltd., Shanghai, China), imaged with a Tannon gel imaging system, and quantified using Image J software. .. Statistical analysis was performed using SPSS 18.0 software.
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